sbh_logo-RED_NoBG
Generic selectors
Exact matches only
Search in title
Search in content
Post Type Selectors

Antibody Therapeutics Development

Expertise in Antibody Engineering & Functional Immunology

With over 15 years of dedicated experience in antibody therapeutics, we support the full spectrum of antibody-based drug discovery—from early target validation to preclinical development. Our team combines deep immunology expertise with practical experience in monoclonal antibodies (mAbs), bispecifics, ADCs, and immune cell engagers to deliver actionable, translationally relevant data.

Comprehensive Antibody Services

  1. Monoclonal Antibody (mAb) Development & Characterization
  • Target Binding Profiling:
    • Affinity measurements (flow cytometry).
    • Cross-reactivity screening 
  • Functional Assessment:
    • Fc effector function: ADCC (NK cell-mediated killing), ADCP (macrophage phagocytosis), CDC (complement activation).
    • Neutralization assays (receptor-ligand blockade)
  • Developability & Safety:
    • Immunogenicity risk assessment (T-cell epitope mapping, ADA screening).
  1. Bispecific Antibodies & Immune Cell Engagers
  • Mechanistic Validation:
    • T-cell redirecting bispecifics (BiTEs, DARTs):
      • Target-dependent T-cell activation (CD69/CD25 expression, IFN-γ release).
      • Tumor cell killing (co-culture with PBMCs or engineered Jurkat reporter cells).
    • Tumor microenvironment (TME) modulation:
      • Checkpoint inhibitor bispecifics (PD-1/CTLA-4, LAG-3/TIM-3).
      • Cytokine-antibody fusions (IL-2/IL-15 payloads).
  1. Antibody-Drug Conjugates (ADCs)
  • Analytical Characterization:
    • Drug-to-antibody ratio (DAR) by HIC-HPLC 
  • Functional Potency:
    • Cell-killing mechanisms: Proliferation inhibition (MTT/XTT), apoptosis (caspase-3/7 activation).
    • Fc-mediated activity: ADCC/ADCP using engineered effector cells (Promega reporter assays).
  • Stability & PK/PD:
    • Serum stability (DAR decay over time).
    • Anti-drug antibody (ADA) assays for immunogenicity.
  1. CAR-T & Cell Therapy Support
  • Target Engagement & Internalization:
    • Flow cytometry (cell surface retention, antigen shedding).
  • Functional Potency:
    • Cytotoxicity 
    • Cytokine storm profiling (IL-6, IFN-γ, TNF-α via MSD/ELISA).
  • Exhaustion & Persistence:
    • Activation/exhaustion markers (PD-1, LAG-3, TIM-3 by flow).
    • Metabolic profiling (Seahorse assays for oxidative phosphorylation/glycolysis).

Advanced Platforms & Assay Systems

  • Primary immune cell models: PBMCs, NK cells, macrophages (THP-1, iPSC-derived).
  • High-throughput screening: agonist/antagonist assays (GPCRs, immune checkpoints).
  • Regulatory-ready assays: GLP-compliant PK/ADA/NAb assays for IND submissions.

Why Work With Us?

  • Depth of experience: 15+ years in antibody engineering, with a focus on translational immunology.
  • Tailored solutions: Custom assay development for novel mechanisms (e.g., microglial activation, phagocytosis).
  • Rigor & reproducibility: All data is generated with clinically relevant endpoints (IC50, EC90, etc.).
  • Collaborative approach: Direct access to scientists who design and execute your studies.