Luminex is a high-throughput immunoassay that enables the simultaneous quantification of multiple analytes in a single sample. SBH Sciences uses Luminex’s xMAP® technology for multiplex detection of up to 500 analytes. We provide custom assay development using both commercial panels and novel biomarkers, optimized for applications in oncology, immunology / inflammation, neurology, and cardiology.
We offer assay development of custom panels from any available commercial assays as well as new innovative biological markers. During the development of the assay, SBH confirms the dynamic range, precision, accuracy / linearity, sensitivity, stability, reproducibility, and specificity of each analyte. SBH ensures the Multiplex compatibility of the panel as well.
Our custom assays can be tailor-made for many applications including:
With plenty of validated assays and extensive experience testing hundreds of analytes, we’re here to support your research needs with accuracy and reliability. Some of the kits we have validated include custom markers from kits produced by MilliporeSigma, R&D Systems, and Thermo Fisher Scientific. Panel sizes range from 4 to 25 analytes.
SBH has tested over 150 distinct analytes on the Luminex. In addition to the wide range of analytes, SBH has tested many different sample matrixes. This includes cell culture supernatant, plasma, serum, urine, CSF, aqueous humor, and others. Additionally, SBH has experience with many different animal species testing on the instrument. Other than human.
The Luminex technology allows for greater multiplexing than any other instrument on the market. Up to 80 distinct target analytes can be tested in a single experiment, reducing the sample, time, and reagents required compared to single analyte testing. The samples are initially loaded onto the plate with magnetic beads and assay buffer. The beads are prebound with antibodies for the targets of interest. The targeted proteins bind to the beads before the sample is washed and the detection antibodies are added, which bind to the proteins of interest. Streptavidin-PE is then bound to the detection antibody. The beads first pass through a red laser, which illuminates the internal dyes to distinguish the microsphere set (detects the beads). Following, a green laser activates the fluorescent reporter dye which determines the result of the assay (detects the signal from the sample-antibody-Streptavidin-PE complex).