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PCR

Our laboratory is equipped with an Applied Biosystems QuantStudio™ 6 Pro Real-Time PCR (qPCR) System. This platform is embedded with RFID readers and has the capability to run TaqMan Array Plated with RFID tags. SBH Sciences can accurately analyze the expression of a group of genes, providing reliable and detailed gene expression data.

QuantStudio™ 6 Pro Real-Time PCR (qPCR) System

SBH Sciences’ qPCR DNA and RNA analysis capabilities

We have extensive experience designing, validating, and performing qPCR assays using TaqMan Array Plates with RFID for precise gene expression profiling. We also have experience with SYBR green detection assays.

Our qPCR capabilities include:

  • RNA and QC extraction
  • cDNA conversion
  • Primer design
  • Validation
  • Reference gene optimization
  • Data analysis
Our innovative SYBR Green primer design and validation service involves optimization for amplicon size and primer specificity using both gel electrophoresis and a melting curve analysis.
Furthermore, we have the ability to perform an analysis of the efficiency of amplification for our designed primer sets.
Our gene expression analysis generates a report detailing differential gene expression across the dataset using the comparative Ct methodology between control and treated samples.

Benefits of Using the QuantStudio™ 6 Pro

Optimize your gene expression analysis with the Applied Biosystems QuantStudio™ 6 Pro. Experience seamless workflows and innovative hands-free operation utilizing smart RFID technology, eliminating manual errors and accelerating your time to results. This platform provides the flexibility to adapt to your diverse research needs. For studies in gene expression, genetic variations, or gene regulation, the QuantStudio™ 6 Pro delivers the accuracy and efficiency you need to advance your research with confidence.

QuantStudio Pro Block Upgrade Kit

How does the qPCR platform work?

The Applied Biosystems QuantStudio™ 6 Pro Real-Time PCR (qPCR) System integrates cutting-edge technology to deliver high-performance gene expression analysis.
The system utilizes real-time monitoring of PCR amplification through fluorescent dye detection, providing precise quantification of target genes.
This platform is embedded with RFID readers, enabling the seamless use of TaqMan Array Plates equipped with RFID tags.
These tags automate data transfer, reducing the need for manual data entry and ensuring accurate assay setup.
The system detects changes in gene expression by measuring the fluorescence intensity during amplification, which directly correlates with the amount of target DNA or RNA present.
SBH Sciences can accurately measure the expression of a group of genes, providing reliable and detailed data that supports your research needs.
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