#001-HIL12P70 In Stock
Interleukin 12 (IL-12) is a regulatory protein produced by activated B lymphocytes and macrophages. The biological activities of IL-12 include the stimulation of growth of activated CD4+ and CD8+T cells and NK cells. Human IL-12 is a disulfide linked heterodimeric protein with a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
Reconstitution: We recommend a quick spin followed by reconstitution in 200 ul of sterile water for injection for 0.5 mg/ml concentration.
Storage: This solution can then be diluted into other aqueous buffers and stored at 4°C for 3 months or below -20°C for future use.
For research Purposes Only. Not for use in diagnostic or therapeutic procedures.
| Properties | |
|---|---|
| Species | Human |
| Expression System | Chinese Hamster Ovary Cells, CHO Cells; Human Embryonic Stem Cells, HEK-293 |
| Molecular Weight | 70 kDa |
| Full Length | p35 subunit: 306 Amino Acids |
| Purity | ≥ 95% by SDS-PAGE and HPLC analyses |
| Tag | No Tag |
| Form | Lyophilized |
| Endotoxin |
<1 EU/µg |
| Activity |
Determined by its ability to stimulate alkaline phosphatase activity of recombinant HEK-293 reporter cells. The ED50 for this effect is < 1.0 ng/ml, corresponding to a specific activity of >1 x 106. |
| Storage | -20°C |